urn:nbn:sciencein.jmns.2019v6.84

Conductive polymer nanocomposite enzyme immobilized biosensor for pesticide detection

  • Priyanka D Virutkar

    Rashtrasant Tukadoji Maharaj Nagpur University

  • Ashish P Mahajan

    Rashtrasant Tukadoji Maharaj Nagpur University

  • Bhavna H Meshram

    Rashtrasant Tukadoji Maharaj Nagpur University

  • Subhash B Kondawar

    Rashtrasant Tukadoji Maharaj Nagpur University

Keywords:

Carbon nanotubes, Acetylcholinesterase,
Pesticides, Biosensors

Abstract

Conductive carbon nanotubes based polyaniline/polypyrrole
polymer nanocomposite (CNT-PANI-PPy) film was electrochemically
synthesized on graphite electrode using cyclic voltammetry. CNT-PANI-PPy
film formed a biocompatible environment to entrap enzyme molecule and
used as biosensor for pesticide detection. This study shows that enzyme
based conductive polymer nanocomposite film itself acts as a mediator.
Carbon nanotubes promote electron transfer reactions in presence of
Acetythiocholine chloride (ATCl) as a substrate at a lower potential and
catalyzed the electrochemical oxidation of enzymatically formed
thiocholine. Surface morphology was studied by scanning electron
microscopy which shows a porous structure of the modified film
beneficial for enzyme immobilization. Electrochemical behavior of the
fabricated electrodes evaluated through cyclic voltammetry and
electrochemical impedance spectroscopy. The detection of pesticide
(acephate) was performed by chronoamperometry and the limit of detection
(LOD) of acephate was found to be 0.007 ppm concentration which is
quite low.

Full text at: http://pubs.thesciencein.org/journal/index.php/jmns/article/view/84

Source:: http://pubs.thesciencein.org/urnnbnsciencein-jmns-2019v6-84/

      

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