Antiproliferative effect of Mosinone-A on expression of apoptotic and cell cycle associated proteins in DMBA induced hamster buccal pouch carcinogenesis
Submitted to: Chemical Biology Letters
Sugunadevi Govindasamy1, Anbu Singaravelu2 and Suresh Kathiresan3
1Assistant Professor, Department of Biochemistry, Dhanalakshmi Srinivasan College of Arts & Science for Women (Autonomous), Perambalur -621212 2Assistant Professor, Department of Biochemistry, Sacred heart college (Autonomous), Thirupattur -635 601 3Associate Professor, Department of Biochemistry& Biotechnology, Annamalai University, Annamalai Nagar.
The present study aimed to investigated the antiproliferative potential of Mosinone-A on cell proliferation, apoptosis and cell cycle proteins during 7, 12-dimethylbenz[a]anthracene (DMBA) induced hamster buccal pouch (HBP) carcinogenesis. A total number of 40 golden Syrian hamsters were randomized into 4 groups of 10 animals in each. Group I animals were served as untreated control. Groups II and III animals were painted with 0.5% DMBA in liquid paraffin three times per week for 14 weeks on the left buccal pouches. Group III animals were orally administered with Mosinone-A (2 mg kg-1 b.wt) starting one week before the exposure to the carcinogen and continued on days alternate to DMBA painting, until the sacrification of the animals. Groups IV animals were received Mosinone-A alone throughout the experimental period. Topical application of DMBA for 14 weeks induced buccal pouch carcinomas associated with increased expression of Cox-2, P21, iNos and SIAT1 whereas decreased expression of Bax, Cleaved caspase-9, P12DOC-1, p16INK4A and p15INK4B. Oral administration of Mosinone-A significantly inhibited the development of HBP carcinomas as revealed by decreased expression of Cox-2, P21, iNos and SIAT1 and over expression Bax, Cleaved caspase-9, P12DOC-1, p16INK4A and p15INK4B. The result of the present study indicates that Mosinone-A can exerts protective effects against DMBA induced buccal pouch carcinogenesis by inhibiting cell proliferation and inducing differentiation and apoptosis.
Key words: Oral cancer; Mosinone-A; DMBA; hamster; carcinogenesis; apoptosis
Note: this manuscript has been removed from journal due to ‘no response from authors’. It was reviewed and accepted by editor for consideration in Chemical Biology Letters, however, authors did not respond to emails and reminders for required revisions and submission of revised/updated manuscript.